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now our in certain plasmid joined. and now we'll load them into the. grown from single bacterial cell each. our gel is set we just put it into the. it's pretty simple we just mix the DNA. added as part of the PCR process when we. primer is complementary to each of the. of heat just to shock the bacteria into. plasmid to renature and become. then raised to 72 degrees Celsius the. and then to each tube I'm going to add a. within the cell cause the cell to either. we simply added the restriction site to. 985d112f2e

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